LOCUS       sequence-103683-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 
            promoter and human codon optimized Cas9 module. Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40-hCas9 L5-L2
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..113
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(139..159)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg ggtagtaatc
      121 tagagtcgcg gccgctcaca ccttcctctt cttcttgggg tcagccctgc tgtctccacc
      181 gagctgagag aggtcgattc ttgtttcata gagccccgta attgactgat gaatcagtgt
      241 ggcgtccagg acctcctttg tagaggtgta ccgctttctg tctatggtgg tgtcgaagta
      301 cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac aagtggataa tgttttctgc
      361 ctgctccctg atgggcttat ccctgtgctt attgtaagca gaaagcacct tatcgaggtt
      421 agcgtcggcg aggatcactc ttttggagaa ttcgcttatt tgctcgatga tctcatcaag
      481 gtagtgtttg tgttgttcca cgaacagctg cttctgctca ttatcttcgg gagacccttt
      541 gagcttttca tagtggctgg ccagatacaa gaaattaacg tatttagagg gcagtgccag
      601 ctcgttacct ttctgcagct cgcccgcact agcgagcatt cgtttccggc cgttttcaag
      661 ctcaaagaga gagtacttgg gaagcttaat gatgaggtc
//
