LOCUS       sequence-103684-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and LacZ module. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 LacZ L5-L2
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..52
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(57..77)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        62..391
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      242..377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(414..436)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 caaattgatg agcaatgctt ttttataatg ccaactttgt atacaaaagt tgctgtggaa
       61 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      121 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
      181 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
      241 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
      301 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
      361 aggctttttt ggaggcctag gcttttgcaa aaagcttcca tgatagatcc cgtcgtttta
      421 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      481 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      541 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
      601 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc
//
