LOCUS       sequence-103686-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express N-terminally FLAG tagged human USP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pRK5-FLAG-USP5
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..38
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    831..842
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 agcttgccgc catggactac aaggacgacg atgacaaggg gtcgacaatg gcggagctga
       61 gtgaggaggc gctgctgtca gtattaccga cgatccgggt ccctaaggct ggagaccggg
      121 tccacaaaga cgagtgcgcc ttctccttcg acacgccgga gtctgagggg ggcctctaca
      181 tctgtatgaa cacgtttctg ggctttggga aacagtatgt ggagagacat ttcaataaga
      241 ccggccagcg agtctacttg cacctccggc ggacccggcg cccgaaagag gaggaccctg
      301 ctacaggcac tggagaccca ccccggaaga agcccacgcg gctggctatt ggtgttgaag
      361 gcgggtttga ccttagcgag gagaagtttg aattagacga ggatgtgaag attgtcattt
      421 tgccagatta cctggagatt gcccgggatg gactgggggg actgcctgac attgtcagag
      481 atcgggtgac cagtgcagtg gaggccctac tgtcggccga ctcagcctcc cgcaagcagg
      541 aggtgcaggc atgggatggg gaagtacggc aggtgtctaa gcatgccttc agcctcaagc
      601 agttggacaa ccctgctcga atccctccct gtggctggaa gtgctccaag tgtgacatga
      661 gagagaacct gtggctcaac ctgactgatg gctccatcct ctgtgggcga cgctacttcg
      721 atggcagtgg gggcaacaac cacgctgtgg agcactaccg agagacaggc tacccgttag
      781 ctgtcaagct gggcaccatc acccctgatg gagctgacgt gtactcatat gatgaggatg
      841 acatggtcct ggaccc
//
