LOCUS       sequence-103687-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and LacZ module. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 LacZ L5-L2
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtagta atctagacct tttagactcg
      121 agcggccgct ttttgacacc agaccaactg gtaatggtag cgaccggcgc tcagctggaa
      181 ttccgccgat actgacgggc tccaggagtc gtcgccacca atccccatat ggaaaccgtc
      241 gatattcagc catgtgcctt cttccgcgtg cagcagatgg cgatggctgg tttccatcag
      301 ttgctgttga ctgtagcggc tgatgttgaa ctggaagtcg ccgcgccact ggtgtgggcc
      361 ataattcaat tcgcgcgtcc cgcagcgcag accgttttcg ctcgggaaga cgtacggggt
      421 atacatgtct gacaatggca gatcccagcg gtcaaaacag gcggcagtaa ggcggtcggg
      481 atagttttct tgcggcccta atccgagcca gtttacccgc tctgctacct gcgccagctg
      541 gcagttcagg ccaatccgcg ccggatgcgg tgtatcgctc gccacttcaa catcaacggt
      601 aatcgccatt tgaccactac catcaatccg gtaggttttc cggctgataa ataaggtttt
      661 cccctgatgc tgccacgcgt gagcggtcgt aatcagcacc gcatcagcaa gtgtatctgc
      721 cgtgcactgc
//
