LOCUS       sequence-103691-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to make gp78 Knockout cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: px330-gp78
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        109..357
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     109..129
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     280..299
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        378..453
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtga gggcctattt
      121 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
      181 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
      241 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
      301 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgcc
      361 cagcctccgc acctacagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      421 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca
      481 agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac aaatggctct
      541 agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      601 ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc aatgggtgga
      661 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      721 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt acatgacctt
      781 atgggacttt cctacttggc agtacatcta cgtatta
//
