LOCUS       sequence-103692-         329 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 
            tet-inducible CMV promoter and multiple cloning site module. 
            Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV/TO R4-R3
FEATURES             Location/Qualifiers
     source          1..329
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..157)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    283..301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tacaggtcac taataccatc taagtagttg attcatagtg actggatatg ttgtgtttta
       61 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      121 ttttacgttt ctcgttcaac tttattatac aaagttgtcc tctagatgca tgctcgagcg
      181 gccgccagtg tgatggatat ctgcagaatt ccagcacact ggcggccgtt actagtggat
      241 ccgagctcgg tacctcgacg atctctatca ctgataggga gatctctatc actgataggg
      301 agagctctgc ttatatagac ctcccaccg
//
