LOCUS       sequence-103694-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human MDM2 (17-125) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pGEX6P2-MDM2 (17-125)
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..32
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(440..462)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(607..625)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatcggatct ggaagttctg ttccaggggc ccctgggatc cagccagatt ccggcaagcg
       61 aacaagaaac cctggttcgt ccgaaaccgc tgctgctgaa actgctgaaa agcgttggtg
      121 cacagaaaga tacctatacc atgaaagagg tgctgtttta tctgggccag tatattatga
      181 ccaaacgcct gtatgatgag aaacagcagc atattgtgta ttgcagcaat gatctgctgg
      241 gtgacctgtt tggtgttccg agctttagcg ttaaagaaca ccgtaaaatc tataccatga
      301 tctatcgtaa tctggtggtg gttaatcagc aagaaagcag cgatagcggc accagcgtta
      361 gcgaaaacta ataactcgag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt
      421 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      481 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      541 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac
      601 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      661 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      781 attg
//
