LOCUS       sequence-103696-         293 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression vector of 53BP1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, 
            Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D
  TITLE     A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and
            BRCA1-CtIP controls DNA repair pathway choice.
  JOURNAL   Mol Cell. 2013 Mar 7;49(5):872-83. doi: 
            10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.
  PUBMED    23333306
REFERENCE   2  (bases 1 to 293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.01.001"; journalName: "Mol Cell"; date: 
            "2013-03-7- 7"; volume: "49"; issue: "5"; pages: "872-83"
COMMENT     Sequence Label: pcDNA5-FRT/T0-Flag-53BP1
FEATURES             Location/Qualifiers
     source          1..293
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     39..63
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             163..186
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctccgga ctctagcgtt taaacttaag cttggtacca tggactacaa agacgatgac
      181 gataaagccg ccagtttaaa cggcgcgcca ttaattaagg atccactagt aacggccgcc
      241 agtgtgctgg aattctgcag atatccatca cactggcggc agctcgagat gga
//
