LOCUS       sequence-103699-         415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9
            fusion protein and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead 
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and 
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. 
            Epub 2014 Oct 9.
  PUBMED    25307932
REFERENCE   2  (bases 1 to 415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date: 
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
COMMENT     Sequence Label: pHR-SFFV-KRAB-dCas9-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggccgcctc tctcggcctt ggtcagattg tcgaacttcc tctgggtaat cagcttggca
       61 ttcagcagct ggcgccagta gttcttcatc ttcttcacga cctcttcgga gggcacgttg
      121 tcgctcttgc cccggttctt gtcgctccga gtcagcactt tgttatcgat ggagtcgtcc
      181 ttcagaaagc tctgaggcac gatagcgtcc acatcgtagt cggacagccg gttgatgtcc
      241 agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta cagcttctcg
      301 ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc cagctctttg
      361 atnccctctt cnatccgctt cattctctcg cggctgttct tctgtccctt ctggg
//
