LOCUS       sequence-103700-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human MDM2 (17-125) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pGEX6P2-MDM2 (17-125)
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(391..414)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(441..463)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gaacgcgcga ggcagatcgg tcagtcagtc acgatgcggc cgctcgagtt attagttttc
       61 gctaacgctg gtgccgctat cgctgctttc ttgctgatta accaccacca gattacgata
      121 gatcatggta tagattttac ggtgttcttt aacgctaaag ctcggaacac caaacaggtc
      181 acccagcaga tcattgctgc aatacacaat atgctgctgt ttctcatcat acaggcgttt
      241 ggtcataata tactggccca gataaaacag cacctctttc atggtatagg tatctttctg
      301 tgcaccaacg cttttcagca gtttcagcag cagcggtttc ggacgaacca gggtttcttg
      361 ttcgcttgcc ggaatctggc tggatcccag gggcccctgg aacagaactt ccagatccga
      421 ttttggagga tggtcgccac caccaaacgt ggcttgccag ccctgcaaag gccatgctat
      481 atacttgctg gatttcaagt acttatcaat ttgtgggata gcttcaatac gttttttaaa
      541 acaaactaat tttgggaacg catccaggca cattgggtcc atgtataaaa caacatcaag
      601 agcgtcatac aacatgaagt caggatgggt tacatgatca ccatttaaat atgttttatg
      661 acataaacga tcttcgaaca ttttcagcat ttcaggtagc ttgctaagaa aatcaacttt
      721 gagagtttca aagtctttac tatatgcaat tctcgaaaca ccgtatctaa tatccaaaac
      781 cgctccttca agcattgaaa tctctgcacg ctct
//
