LOCUS       sequence-103703-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo homologous recombination substrate composed of two 
            differentially mutated GFP genes oriented as direct repeats and 
            separated by a drug selection marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Pierce AJ, Johnson RD, Thompson LH, Jasin M
  TITLE     XRCC3 promotes homology-directed repair of DNA damage in mammalian 
            cells.
  JOURNAL   Genes Dev. 1999 Oct 15. 13(20):2633-8.
  PUBMED    10541549
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1999 Oct 15. 13(20):2633-8."
COMMENT     Sequence Label: pDRGFP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..51
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             73..93
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      307..316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(358..379)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(619..638)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agatccgccg ccactatggg atcaagatcg ccaaaaaaga agagaaaggt gccgaagaag
       61 catgcagcac caccaaaaaa aaaacgaaaa gtagaagacc cacgaggcaa caccagcggc
      121 gtgctgagca cccccaaggc caagagggcc aagcaccccc ccggcaccga gaagcccagg
      181 agcaggagcc agagcgagca gcccgccacc tgccccatct gctacgccgt gatcaggcag
      241 agcaggaacc tgaggaggca cctggagctg aggcacttcg ccaagcccgg cgtggatcca
      301 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      361 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcta gggataacag
      421 ggtaatacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      481 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      541 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      601 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      661 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      721 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      781 aagcagaaga acggcatcaa ggtgaacttc aa
//
