LOCUS       sequence-103706-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter for shRNA expression. Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK L1-R5
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    375..498
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(553..572)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(554..572)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(577..593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(577..593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(695..714)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(773..792)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tctatcgatc tagtgctgca gtatttagca tgccccaccc
      121 atctgcaagg cattctggat agtgtcaaaa cagccggaaa tcaagtccgt ttatctcaaa
      181 ctttagcatt ttgggaataa atgatatttg ctatgctggt taaattagat tttagttaaa
      241 tttcctgctg aagctctagt acgataagta acttgaccta agtgtaaagt tgagatttcc
      301 ttcaggttta tatagcttgt gcgccgcctg ggtacctcgg tcttcacaga tctagagctc
      361 ttttttgaat tcgacaactt tgtatacaaa agttgaacga gaaacgtaaa atgatataaa
      421 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
      481 acatatccag tcactatgaa tcaactactt agatggtatt agtgacctgt actgcagggc
      541 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
      601 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      661 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
      721 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
      781 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta t
//
