LOCUS       sequence-103711-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and sgRNA scaffold module for gRNA expression. Compatible 
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(175..423)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(233..252)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(403..423)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     protein_bind    467..590
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
       61 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
      121 ctgcgatgag tggcagggcg gggcgtaaag atctggatca ctcacctgca caccggtgtt
      181 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
      241 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      301 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattccaat
      361 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      421 ctcggtgaag ggggcggccg ctcgaggcta gtctcgtgat cgatagcaac tttgtataga
      481 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
      541 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg aatcaacta
//
