LOCUS       sequence-103714-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human MDM2 (1-120) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pGEX6P2-MDMX (1-120)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(436..459)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(486..508)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gaacgcgcga ggcagatcgg tcagtcagtc acgatgcggc cgctcgagtc gacccgggaa
       61 ttcttagaga gtctgagcag catctgtagt agcagtggct aaagtgacaa gattctttct
      121 tagcatatca tagagagggc ttgggtcttt cacggagaag ctctgacgtc ccagtagttc
      181 tcccaaaaga tctccaccac aatataccat atgctgctcc tgctgatcat aaagttgctt
      241 caccattatg tactgaccta aatagtgcat gacctcttta acagtgaaca tttcaccttg
      301 cgcacctgct gcatgcaaaa tcttcaaaag cggcagtttt ggtcgtacct gattgatttg
      361 tccaggagag atcctgcaag cactgtcaga tgttgaacac tgagcagagg tggaaaatga
      421 tgtcatggat cccaggggcc cctggaacag aacttccaga tccgattttg gaggatggtc
      481 gccaccacca aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt
      541 caagtactta tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg
      601 gaacgcatcc aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat
      661 gaagtcagga tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc
      721 gaacattttc agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc
      781 tttactatat gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat
      841 tgaaatctct gcac
//
