LOCUS       sequence-103720-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human MDM4 (residues 23-111) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pGEX6P2-MDMX (23-111)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..35
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(380..402)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(547..565)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        633..737
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctcaatgga tctggaagtt ctgttccagg ggcccctggg atcccagatt aatcaggttc
       61 gtccgaaact gccgctgctg aaaattctgc atgcagccgg tgcacagggt gaaatgttta
      121 ccgttaaaga agtgatgcat tatctgggcc agtacattat ggtgaaacag ctgtatgatc
      181 agcaagaaca gcatatggtt tattgtggtg gtgatctgct gggtgaactg ctgggtcgtc
      241 agagctttag cgttaaagat ccgagtccgc tgtatgatat gctgcgtaaa aatctggtta
      301 ccctggcaac ctaactcgag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt
      361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      421 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      481 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac
      541 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      601 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      661 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaa
//
