LOCUS       sequence-103724-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human MDM4 (residues 23-111) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pGEX6P2-MDMX (23-111)
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(326..349)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(376..398)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgcgcgaggc agaatcgtca gtcagtcacg atgcggccgc ctcgagttag gttgccaggg
       61 taaccagatt tttacgcagc atatcataca gcggactcgg atctttaacg ctaaagctct
      121 gacgacccag cagttcaccc agcagatcac caccacaata aaccatatgc tgttcttgct
      181 gatcatacag ctgtttcacc ataatgtact ggcccagata atgcatcact tctttaacgg
      241 taaacatttc accctgtgca ccggctgcat gcagaatttt cagcagcggc agtttcggac
      301 gaacctgatt aatctgggat cccaggggcc cctggaacag aacttccaga tccgattttg
      361 gaggatggtc gccaccacca aacgtggctt gccagccctg caaaggccat gctatatact
      421 tgctggattt caagtactta tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa
      481 ctaattttgg gaacgcatcc aggcacattg ggtccatgta taaaacaaca tcaagagcgt
      541 catacaacat gaagtcagga tgggttacat gatcaccatt taaatatgtt ttatgacata
      601 aacgatcttc gaacattttc agcatttcag gtagcttgct aagaaaatca actttgagag
      661 tttcaaagtc tttactatat gcaattctcg aaacaccgta tctaatatcc aaaaccgctc
      721 cttcaagcat tgaaatctct gcacgctctt ttggacaacc acccaacatg ttgtgcttgt
      781 cagctatata acgtatgatg gccatagact gtgttaattt aacatcac
//
