LOCUS       sequence-103729-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pST1374
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(27..407)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..110)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     301..320
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    453..677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     666..683
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(706..723)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(733..774)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
       61 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
      121 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
      181 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
      241 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
      301 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
      361 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtggg gataccccct
      421 agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg
      481 cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat
      541 agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt
      601 gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg
      661 gcaactagaa ggcacagtcg aggctgatca gcgggtttaa actcaatggt gatggtgatg
      721 atgaccggta cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa
      781 gggcccttaa aagtttatct cgccgttatt aaatttccgt ctcacttcct ctaaggttaa
      841 tgtgccggct ttaatcattt ctccaccaat taaaagctct tctacactaa gaacagctcc
      901 attacaatta gtgatatgat ttaatcgtgt aagctgagct ttgtagtttc ctttaaagtg
      961 accactcaca aataaaaact taaattccg
//
