LOCUS       sequence-103732-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH
  TITLE     Drosophila Brainbow: a recombinase-based fluorescence labeling 
            technique to subdivide neural expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):253-9. doi: 10.1038/nmeth.1566. Epub 2011
            Feb 6.
  PUBMED    21297621
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1566"; journalName: "Nat Methods"; date: "2011-03"; 
            volume: "8"; issue: "3"; pages: "253-9"
COMMENT     Sequence Label: dBrainbow
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..51
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             773..814
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 acgtaagcta gggtaccata acttcgtata atgtatgcta tacgaagtta tcaaaatgat
       61 accggtcgga gtaagcaaag gtgaggagag cgtgatcaaa cccgagatga agatgcgcta
      121 ctatatggac ggcagcgtca acggccacga gtttaccatc gagggcgagg gcacgggacg
      181 cccgtacgaa ggacaccagg agatgaccct gcgcgtgacc atggccgagg gtggcccgat
      241 gcccttcgcc ttcgatctgg tgagccacgt gttctgctac ggtcaccgcg tcttcaccaa
      301 ataccccgag gaaatccccg attacttcaa gcaagccttc ccggagggcc tgtcgtggga
      361 gcgcagcctg gagttcgagg atggcggaag cgccagcgtg agcgcgcaca tcagcctgcg
      421 gggcaacacc ttctaccata agagcaagtt caccggcgtg aatttcccag ccgatggccc
      481 catcatgcag aaccagagtg tggattggga gcccagcacc gagaagatca ccgcaagcga
      541 tggcgtgctg aagggcgatg ttaccatgta cctgaagctg gagggaggcg gcaaccacaa
      601 gtgccagatg aaaaccacct acaaggccgc caaggggatc ctggagatgc ccggagatca
      661 ctacattggc caccgcctgg tccgcaagac agaaggaaac atcaccgagc aggtggagga
      721 tgccgtggcg cattcgggca tggatgagct gtacaagggc gcgccagcta atggcaagcc
      781 catccccaac cccctgctgg gcctggatag cacctgacgt aagctagtct agaggatctt
      841 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
      901 aagctctaag gtaaa
//
