LOCUS       sequence-103733-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH
  TITLE     Drosophila Brainbow: a recombinase-based fluorescence labeling 
            technique to subdivide neural expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):253-9. doi: 10.1038/nmeth.1566. Epub 2011
            Feb 6.
  PUBMED    21297621
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1566"; journalName: "Nat Methods"; date: "2011-03"; 
            volume: "8"; issue: "3"; pages: "253-9"
COMMENT     Sequence Label: dBrainbow
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             141..182
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          279..344
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             474..494
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    766..847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(803..822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aggggatcct ggagatgccc ggagatcact acattggcca ccgcctggtc cgcaagacag
       61 aaggaaacat caccgagcag gtggaggatg ccgtggcgca ttcgggcatg gatgagctgt
      121 acaagggcgc gccagctaat ggcaagccca tccccaaccc cctgctgggc ctggatagca
      181 cctgacgtaa gctagtctag aggatctttg tgaaggaacc ttacttctgt ggtgtgacat
      241 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
      301 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
      361 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
      421 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
      481 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
      541 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
      601 ctgcactgct atacaagaaa attatggaaa aatatttgat gtatagtgcc ttgactagag
      661 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      721 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      781 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      841 tcactgcatt ctagttgtgg tttgtccaa
//
