LOCUS       sequence-103734-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and sgRNA scaffold module for gRNA expression. Compatible 
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        125..373
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     125..145
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     296..315
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tctatcgatc acgagactag cctcgagcgg ccgccccctt
      121 caccgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
      181 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
      241 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      301 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      361 aggacgaaac accggtgtgc aggtgagtga tccagatctt tacgccccgc cctgccactc
      421 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      481 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
      541 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
      601 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
      661 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactg
//
