LOCUS       sequence-103754-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express C-terminally FLAG tagged human gp78.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pCDNA-gp78-FLAG RING mutant C356G H361A
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cgggccctct agactcgagc ggccgccact gtgctggata
      121 tctgcagaat tcggcttgcc gccatgccgc tgctcttcct cgagcgcttc ccctggccca
      181 gcctccgcac ctacacgggc ctcagcggcc tggccctgct gggcaccatc atcagcgcct
      241 accgcgcgct cagccagccc gaggccggcc ccggcgagcc ggaccagcta acggcctcgc
      301 tgcagcctga gccgccggcg cccgcccggc cgagcgccgg gggaccccgg gcccgcgatg
      361 tggcccagta cctgctctca gacagcctct tcgtgtgggt tctagtaaat accgcttgct
      421 gtgttttgat gttggtggct aagctcatcc agtgtattgt gtttggccct cttcgagtga
      481 gtgagagaca gcatctcaaa gacaaatttt ggaattttat tttctacaag ttcattttca
      541 tctttggtgt gctgaatgtc cagacagtgg aggaggtggt catgtggtgc ctctggtttg
      601 ccggacttgt ctttctgcac ctgatggttc agctctgcaa ggatcgattt gaatatcttt
      661 ccttctcgcc caccacgctg atgagcagcc acggtcgagt cctgtccctg ttggttgcca
      721 tgctgctttc ctgctgtgga ctggcggccg tctgctccat caccggctac acccacggaa
      781 tgcacacctt ggctttcatg gctgcagagt ctcttcttgt gacagtgagg actgctcatg
      841 tgattttac
//
