LOCUS       sequence-103757-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PPAR reporter.  3xDR1 sites upstream of a luciferase reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Kim JB, Wright HM, Wright M, Spiegelman BM
  TITLE     ADD1/SREBP1 activates PPARgamma through the production of endogenous
            ligand.
  JOURNAL   Proc Natl Acad Sci U S A 1998 Apr 14;95(8):4333-7.
  PUBMED    9539737
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1998-04-14"; volume: "95(8)"; pages: 
            "4333-7"
COMMENT     Sequence Label: PPRE X3-TK-luc
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          238..303
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             433..453
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    878..1012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(915..934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     969..988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1030..1046)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1030..1046)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1043..1065)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1054..1070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
       61 gagagatcct cataaaggcc aagaagggcg gaaagtccaa attgtaaaat gtaactgtat
      121 tcagcgatga cgaaattctt agctattgta atactctaga ggatctttgt gaaggaacct
      181 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
      241 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
      301 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
      361 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
      421 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
      481 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
      541 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
      601 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
      661 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
      721 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
      781 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      841 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgggaattc
     1021 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1081 catacgagcc ggaa
//
