LOCUS       sequence-103758-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PPAR reporter.  3xDR1 sites upstream of a luciferase reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Kim JB, Wright HM, Wright M, Spiegelman BM
  TITLE     ADD1/SREBP1 activates PPARgamma through the production of endogenous
            ligand.
  JOURNAL   Proc Natl Acad Sci U S A 1998 Apr 14;95(8):4333-7.
  PUBMED    9539737
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1998-04-14"; volume: "95(8)"; pages: 
            "4333-7"
COMMENT     Sequence Label: PPRE X3-TK-luc
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(103..165)
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     primer_bind     complement(388..405)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(388..404)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(397..419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(932..951)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agaatggcgc cgggcctttc tttatgtttt tggcgtcttc cattttacca acagtaccgg
       61 aatgccaagc tcctcgagat ctgcggcacg ctgttgacgc tgttaagcgg gtcgctgcag
      121 ggtcgctcgg tgttcgaggc cacacgcgtc accttaatat gcgaagtgga cctcggaccg
      181 cgccgccccg actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggcc
      241 ggatcctcta gagtcgactc ccgaacgtga cctttgtcct ggtcccctgt cgactcccga
      301 acgtgacctt tgtcctggtc ccctgtcgac tcccgaacgt gacctttgtc ctggtcccct
      361 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
      421 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      481 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      541 atggcgattc cgttgcaatg gctggcggta atattgttct ggatattacc agcaaggccg
      601 atagtttgag ttcttctact caggcaagtg atgttattac taatcaaaga agtattgcga
      661 caacggttaa tttgcgtgat ggacagactc ttttactcgg tggcctcact gattataaaa
      721 acacttctca ggattctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt
      781 ttagctcccg ctctgattct aacgaggaaa gcacgttata cgtgctcgtc aaagcaacca
      841 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      901 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      961 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1021 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1081 gg
//
