LOCUS       sequence-103760-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PPAR reporter.  3xDR1 sites upstream of a luciferase reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Kim JB, Wright HM, Wright M, Spiegelman BM
  TITLE     ADD1/SREBP1 activates PPARgamma through the production of endogenous
            ligand.
  JOURNAL   Proc Natl Acad Sci U S A 1998 Apr 14;95(8):4333-7.
  PUBMED    9539737
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1998-04-14"; volume: "95(8)"; pages: 
            "4333-7"
COMMENT     Sequence Label: PPRE X3-TK-luc
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    14..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(573..593)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          723..788
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 attacgaatt cccgatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      181 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      241 ggctgattat gatctctagt caaggcacta tacatcaaat attccttatt aaccccttta
      301 caaattaaaa agctaaaggt acacaatttt tgagcatagt tattaatagc agacactcta
      361 tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat aactgttatg cctacttata
      421 aaggttacag aatatttttc cataattttc ttgtatagca gtgcagcttt ttcctttgtg
      481 gtgtaaatag caaagcaagc aagagttcta ttactaaaca cagcatgact caaaaaactt
      541 agcaattctg aaggaaagtc cttggggtct tctacctttc tcttcttttt tggaggagta
      601 gaatgttgag agtcagcagt agcctcatca tcactagatg gcatttcttc tgagcaaaac
      661 aggttttcct cattaaaggc attccaccac tgctcccatt catcagttcc ataggttgga
      721 atctaaaata cacaaacaat tagaatcagt agtttaacac attatacact taaaaatttt
      781 atatttacct tagagcttta aatctctgta ggtagtttgt ccaattatgt cacaccacag
      841 aagtaaggtt ccttcacaaa gatcctctag agtattacaa tagctaagaa tttcgtcatc
      901 gctgaataca gttacatttt acaatttgga ctttccgccc ttcttggcct ttatgaggat
      961 ctctctgatt tttcttgcgt cgagttttcc ggtaagacct ttcggtactt cgtccacaaa
     1021 cacaactcct ccgcgcaact ttttcgcggt tgttacttga ctggcgacgt aatccacgat
     1081 ctcttttt
//
