LOCUS       sequence-103761-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression TALEN vector with N152, C+18 truncations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Gan Z, Ding L, Burckhardt CJ, Lowery J, Zaritsky A, Sitterley K, 
            Mota A, Costigliola N, Starker CG, Voytas DF, Tytell J, Goldman RD, 
            Danuser G
  TITLE     Vimentin Intermediate Filaments Template Microtubule Networks to 
            Enhance Persistence in Cell Polarity and Directed Migration.
  JOURNAL   Cell Syst. 2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 
            10.1016/j.cels.2016.08.007.
  PUBMED    27667364
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 
            10.1016/j.cels.2016.08.007."
COMMENT     Sequence Label: pCGS652
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    603..624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(639..644,645..662,663..669)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    677..693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     682..704
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     701..717
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     701..717
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             713..1000
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPC"
     primer_bind     736..756
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        738..756
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(803..822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(804..822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(832..849)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(832..848)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(841..863)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttcctcaagt gtcaatgtac cagctttaat catttctcca ccaatcaaaa gctcttctac
       61 actaagaaca gctccattac aattagtgat atgattcaat cttgtaagct gagctttgta
      121 gtttcctttg aaatgaccac tcacaaacaa gaacttaaat tctgttactg aagatggata
      181 gactttccac cattcattag ggttgatatg cttgtttctt gtttgattct cttcgacata
      241 tctttgcatt tcatctgctt gaccaattgg aagattataa cctcctgaat atgccttagt
      301 atcaacgatc acaccgtaat caataggaga tccgacagta taaattgctc cgtctggttt
      361 ccttgatcca cccaaatgtt taccacgata accataaacc ttcataaaga actccatcac
      421 cttcatttca aggattctat cctgagttga atttcttgcg atttcaatca attcaatata
      481 ttcatgtggc acatatttca atttatgtct aagttcagat ttcttctctt ccaattcaga
      541 tttcactagc tgggatccgt tggagacgac cggtggaaag cgggcagtga gcgcaacgca
      601 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      661 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      721 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga ggacctcgag
      781 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
      841 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      901 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      961 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt a
//
