LOCUS       sequence-103762-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express C-terminal Ubl4A K48R, for making the stable cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pcDNA 5/FR/TO-Ubl4A K48R
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             656..685
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             704..727
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(824..841)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt gccgccatgc agctgacggt
      181 gaaggcgctg cagggccgcg agtgcagcct gcaggtgcca gaggacgagc tggtgtccac
      241 gctgaagcag ctggtctccg agaagctgaa cgtcccagtg cgccagcagc ggctgctgtt
      301 caagggcagg gccctggcag atgggaaacg actctcggat tatagcatcg ggcccaactc
      361 caagctcaac ctagtggtca aacccctgga gaaggtgcta ctagaagaag gcgaggccca
      421 gaggctggcc gactccccac ccccgcaggt ctggcagctg atctccaaag tcttggcccg
      481 ccacttcagt gcggcagatg ccagcagggt cctggaacag ctacagaggg attacgagag
      541 gtccctgagt cgcctgacgc tggacgacat cgaacggttg gccagccgct tcctgcaccc
      601 tgaagtgact gagacaatgg agaagggctt ctccaaaacg cgtacgcggc cgctcgagca
      661 gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca aggatgacga
      721 cgataagtag ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg
      781 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
      841 agttgccagc catctgttgt t
//
