LOCUS       sequence-103763-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression TALEN vector with N152, C+18 truncations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Gan Z, Ding L, Burckhardt CJ, Lowery J, Zaritsky A, Sitterley K, 
            Mota A, Costigliola N, Starker CG, Voytas DF, Tytell J, Goldman RD, 
            Danuser G
  TITLE     Vimentin Intermediate Filaments Template Microtubule Networks to 
            Enhance Persistence in Cell Polarity and Directed Migration.
  JOURNAL   Cell Syst. 2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 
            10.1016/j.cels.2016.08.007.
  PUBMED    27667364
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 
            10.1016/j.cels.2016.08.007."
COMMENT     Sequence Label: pCGS652
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..94
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             95..121
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             complement(425..832)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     682..704
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     696..713
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     697..713
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     723..742
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        723..741
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(789..809)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(789..807)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(828..844)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(828..844)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(841..863)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    852..868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(876..882,883..900,901..906))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 agctaggatc taactcgaga aagatattgt atatatcgta acaataggag gttcaacaat
       61 ggcttcctcc cctccaaaga aaaagagaaa ggttagttgg aaggacgcaa gtggttggtc
      121 tagagtggat ctacgcacgc tcggctacag tcagcagcag caagagaaga tcaaaccgaa
      181 ggtgcgttcg acagtggcgc agcaccacga ggcactggtg ggccatgggt ttacacacgc
      241 gcacatcgtt gcgctcagcc aacacccggc agcgttaggg accgtcgctg tcacgtatca
      301 gcacataatc acggcgttgc cagaggcgac acacgaagac atcgttggcg tcggcaaaca
      361 gtggtccggc gcacgcgccc tggaggcctt gctcacggat gcgggggagt tgagaggtcc
      421 gccgttacag ttggacacag gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc
      481 aatggaggca gtgcatgcat cgcgcaatgc actgacgggt gcccccctgg agacgggcgc
      541 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
      601 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
      661 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
      721 cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc
      781 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
      841 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      901 tgtaaagcct ggggtgccta atgagtgagc taactca
//
