LOCUS       sequence-103765-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H
  TITLE     Solution structure of DnaE intein from Nostoc punctiforme: 
            structural basis for the design of a new split intein suitable for 
            site-specific chemical modification.
  JOURNAL   FEBS Lett. 2009 May 6;583(9):1451-6. doi: 
            10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1.
  PUBMED    19344715
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.febslet.2009.03.058"; journalName: "FEBS Lett"; date: 
            "2009-05-6- 6"; volume: "583"; issue: "9"; pages: "1451-6"
COMMENT     Sequence Label: pHYBAD94
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             296..313
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(353..370)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      523..609
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      701..728
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgggcttc atagcttcta attgtttcaa
      121 tggtacccag tacaaactta tcctgaacgg taaaaccctg aaaggtgaaa ccaccaccga
      181 agctgttgac gctgctaccg cggaaaaagt tttcaaacag tacgctaacg acaacggtgt
      241 tgacggtgaa tggacctacg acgacgctac caaaacctac accgtaacgg aattccatca
      301 tcatcatcat cattaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
      361 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
      421 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
      481 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      541 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      601 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
      661 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
      721 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
      781 tc
//
