LOCUS       sequence-103767-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H
  TITLE     Solution structure of DnaE intein from Nostoc punctiforme: 
            structural basis for the design of a new split intein suitable for 
            site-specific chemical modification.
  JOURNAL   FEBS Lett. 2009 May 6;583(9):1451-6. doi: 
            10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1.
  PUBMED    19344715
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.febslet.2009.03.058"; journalName: "FEBS Lett"; date: 
            "2009-05-6- 6"; volume: "583"; issue: "9"; pages: "1451-6"
COMMENT     Sequence Label: pHYBAD94
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             240..262
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(289..573)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(347..366)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gccaagctta atgatgatga tgatgatgga attccgttac ggtgtaggtt ttggtagcgt
       61 cgtcgtaggt ccattcaccg tcaacaccgt tgtcgttagc gtactgtttg aaaacttttt
      121 ccgcggtagc agcgtcaaca gcttcggtgg tggtttcacc tttcagggtt ttaccgttca
      181 ggataagttt gtactgggta ccattgaaac aattagaagc tatgaagccc atatgtatat
      241 ctccttctta aagttaaaca aaattatttc tagcccaaaa aaacgggtat ggagaaacag
      301 tagagagttg cgataaaaag cgtcaggtag gatccgctaa tcttatggat aaaaatgcta
      361 tggcatagca aagtgtgacg ccgtgcaaat aatcaatgtg gacttttctg ccgtgattat
      421 agacactttt gttacgcgtt tttgtcatgg ctttggtccc gctttgttac agaatgcttt
      481 taataagcgg ggttaccggt ttggttagcg agaagagcca gtaaaagacg cagtgacggc
      541 aatgtctgat gcaatatgga caattggttt cttctctgaa tggcgggagt atgaaaagta
      601 tggctgaagc gcaaaatgat cccctgctgc cgggatactc gtttaatgcc catctggtgg
      661 cgggtttaac gccgattgag gccaacggtt atctcgattt ttttatcgac cgaccgctgg
      721 gaatgaaagg ttatattctc aatctcacca ttcgcgg
//
