LOCUS       sequence-103770-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdN38 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-467
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             629..651
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(736..755)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 aaacagccaa gcttaatgat gatgatgatg atggaattcc gttacggtgt aggttttggt
       61 agcgtcgtcg taggtccatt caccgtcaac accgttgtcg ttagcgtact gtttgaaaac
      121 tttttccgcg gtagcagcgt caacagcttc ggtggtggtt tcacctttca gggttttacc
      181 gttcaggata agtttgtact gggtacctag ctgagtatta tggagaacaa gtccattggg
      241 agctatgaag ttgtgggttt ccggaattac aaggtcgtag acccagttat tgtaatctat
      301 tatccttacc gaggaaacct catggaattc aagttctcct ttggatatta ttcttcttct
      361 cagtacctct tccctaacac caacaacaac atctccaggt ttaacttcag cagcggggac
      421 ccattgcagg ccatctctaa agaggagaac tgggtgagag ggagtaacct taagggagta
      481 tccactggat agttttatct caatgagctt ctcaaccttc tcgcgataga tgtatgaagc
      541 cctcgttttc ttaatttcac cactagcgat gtccaacgtg tacacaaaga cattatctaa
      601 tggaactgcg taaccattca tatgtatatc tccttcttaa agttaaacaa aattatttct
      661 agcccaaaaa aacgggtatg gagaaacagt agagagttgc gataaaaagc gtcaggtagg
      721 atccgctaat cttatggata aaaatgctat ggcatagcaa agtgtgacgc cgtgcaaata
      781 atcaatgtgg acttttctgc cgtgattata gacacttttg ttacg
//
