LOCUS       sequence-103773-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAC7 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pHYBAD177
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             299..316
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(356..373)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      526..612
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      704..731
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgggactt ctcgtacttc acaacacggt
      121 tataggtacc cagtacaaac ttatcctgaa cggtaaaacc ctgaaaggtg aaaccaccac
      181 cgaagctgtt gacgctgcta ccgcggaaaa agttttcaaa cagtacgcta acgacaacgg
      241 tgttgacggt gaatggacct acgacgacgc taccaaaacc tacaccgtaa cggaattcca
      301 tcatcatcat catcattaag cttggctgtt ttggcggatg agagaagatt ttcagcctga
      361 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
      421 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
      481 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
      541 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      601 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
      661 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
      721 gatggccttt ttgcgtttct
//
