LOCUS       sequence-103774-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TvoVMAC7 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pHYBAD177
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..27)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             242..264
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(291..575)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(349..368)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gccagcttaa tgatgatgat gatgatggaa ttccgttacg gtgtaggttt tggtagcgtc
       61 gtcgtaggtc cattcaccgt caacaccgtt gtcgttagcg tactgtttga aaactttttc
      121 cgcggtagca gcgtcaacag cttcggtggt ggtttcacct ttcagggttt taccgttcag
      181 gataagtttg tactgggtac ctataaccgt gttgtgaagt acgagaagtc ccatatgtat
      241 atctccttct taaagttaaa caaaattatt tctagcccaa aaaaacgggt atggagaaac
      301 agtagagagt tgcgataaaa agcgtcaggt aggatccgct aatcttatgg ataaaaatgc
      361 tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt
      421 atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt acagaatgct
      481 tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga cgcagtgacg
      541 gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga gtatgaaaag
      601 tatggctg
//
