LOCUS       sequence-103776-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express N-terminally GST tagged human USP13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pet42c(+)-GST-USP13
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..60
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             76..120
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             151..162
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actagtggtt ctggtcatca ccatcaccat cactccgcgg gtctggtgcc acgcggtagt
       61 actgcaattg gtatgaaaga aaccgctgct gctaaattcg aacgccagca catggacagc
      121 ccagatctgg gtaccggtgg tggctccggt attgagggac gcgggtccat ggcgatatcg
      181 gggatccgaa ttctgtacag gccttggcgc gcctgcaggc gagctccgtc gacaatgcag
      241 cgccggggcg ccctgttcgg catgccgggc ggcagcggag gcaggaagat ggctgcagga
      301 gacatcggcg agctgctagt gccccacatg cccacgatcc gcgtgcccag gtccggcgac
      361 agggtctaca agaacgagtg cgccttctcc tacgactctc ccaattctga aggtggactc
      421 tatgtatgca tgaatacatt tttggccttt ggaagggaac atgttgaaag acattttcga
      481 aaaactggac agagtgtata catgcacctg aaaagacatg tgcgagagaa ggtaagaggg
      541 gcgtctggtg gagcgttacc aaaaaggagg aattccaaga tttttttaga tctagatact
      601 gatgacgatt taaatagcga cgattatgaa tatgaagatg aagccaaact tgttatattc
      661 ccagatcact atgaaatagc actaccaaat attgaggagt taccagccct ggtaacaatt
      721 gcttgtgatg cagttctcag ctcaaaatct ccatacagaa agcaggaccc agacacgtgg
      781 gaaa
//
