LOCUS       sequence-103777-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express N-terminally GST tagged human USP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pet42c(+)-GST-USP5
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..60
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             76..120
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             151..162
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actagtggtt ctggtcatca ccatcaccat cactccgcgg gtctggtgcc acgcggtagt
       61 actgcaattg gtatgaaaga aaccgctgct gctaaattcg aacgccagca catggacagc
      121 ccagatctgg gtaccggtgg tggctccggt attgagggac gcgggtccat ggcgatatcg
      181 gggatccgaa ttctgtacag gccttggcgc gcctgcaggc gagctccgtc gacaatggcg
      241 gagctgagtg aggaggcgct gctgtcagta ttaccgacga tccgggtccc taaggctgga
      301 gaccgggtcc acaaagacga gtgcgccttc tccttcgaca cgccggagtc tgaggggggc
      361 ctctacatct gtatgaacac gtttctgggc tttgggaaac agtatgtgga gagacatttc
      421 aataagaccg gccagcgagt ctacttgcac ctccggcgga cccggcgccc gaaagaggag
      481 gaccctgcta caggcactgg agacccaccc cggaagaagc ccacgcggct ggctattggt
      541 gttgaaggcg ggtttgacct tagcgaggag aagtttgaat tagacgagga tgtgaagatt
      601 gtcattttgc cagattacct ggagattgcc cgggatggac tggggggact gcctgacatt
      661 gtcagagatc gggtgaccag tgcagtggag gccctactgt cggccgactc agcctcccgc
      721 aagcaggagg tgcaggcatg ggatggggaa gtacggcagg tgtctaagca tgccttcagc
      781 ctcaagcagt tggacaaccc tgctcgaatc cctccctgtg gctggaagtg ctccaagtgt
      841 gacatgagag ag
//
