LOCUS       sequence-103778-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pCMV-Ubl4A K48R
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             586..615
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             634..657
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(677..693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(677..693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtgaaccgtc agaattttgt aatacgactc actatagggc ggccgggaat tcgtcgactg
       61 gatccggtac cgaggagatc tgccgccgcg atcgccatgc agctgacggt gaaggcgctg
      121 cagggccgcg agtgcagcct gcaggtgcca gaggacgagc tggtgtccac gctgaagcag
      181 ctggtctccg agaagctgaa cgtcccagtg cgccagcagc ggctgctgtt caagggcagg
      241 gccctggcag atgggaaacg actctcggat tatagcatcg ggcccaactc caagctcaac
      301 ctagtggtca aacccctgga gaaggtgcta ctagaagaag gcgaggccca gaggctggcc
      361 gactccccac ccccgcaggt ctggcagctg atctccaaag tcttggcccg ccacttcagt
      421 gcggcagatg ccagcagggt cctggaacag ctacagaggg attacgagag gtccctgagt
      481 cgcctgacgc tggacgacat cgaacggttg gccagccgct tcctgcaccc tgaagtgact
      541 gagacaatgg agaagggctt ctccaaaacg cgtacgcggc cgctcgagca gaaactcatc
      601 tcagaagagg atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt
      661 taaacggccg gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga
      721 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
      781 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatgg
//
