LOCUS       sequence-103789-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NanoLuc luciferase with a nuclear localization signal (NLS) driven 
            by MET17 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB648
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(397..584)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     505..529
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gcgcgcgtaa tacgactcac tatagggcga attgggtacc ggccgcaaat taaagccttc
       61 gagcgtccca aaaccttctc aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt
      121 ctgtgagggg agcgtttccc tgctcgcagg tctgcagcga ggagccgtaa tttttgcttc
      181 gcgccgtgcg gccatcaaaa tgtatggatg caaatgatta tacatgggga tgtatgggct
      241 aaatgtacgg gcgacagtca catcatgccc ctgagctgcg cacgtcaaga ctgtcaagga
      301 gggtattctg ggcctccatg tcgctggccg ggtgacccgg cggggacgag gcaagctaaa
      361 cagatctata ttaccctgtt atccctagcg gatctgccgg tagaggtgtg gtcaataaga
      421 gcgacctcat actatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaat
      481 aagaattttc gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt
      541 ataacttatt taataataaa aatcataaat cataagaaat tcgcttattt agaagtggcg
      601 cgccttattt gtacaatgat cccccacccg ccagaatgcg ttcgcacagc cgccagccgg
      661 tcactccgtt gatggttact cggaacagca gggagccgtc ggggttgatc aggcgctcgt
      721 cgataatttt gttgccgttc cacagggtcc ctgttacagt gatctttttg ccgtcgaaca
      781 cggcgatgcc ttcatacggc cgtccgaaat agtcgatcat gttcggcgta accccgtcga
      841 ttaccagtgt gccatagtgc aggatcacct taaagtgatg atcatccaca gggtacacca
      901 ccttaaaaat tttttcgatc tggcccattt ggtcgccgct cagaccttca tacgggatga
      961 tgacatggat gtcgatcttc agcccatttt caccgctcag gacaatcctt tggatcggag
     1021 ttacggacac cccgagattc tgaaacaaac tggacacacc tccctgttca aggacttggt
     1081 ccaggttgta gccggct
//
