LOCUS       sequence-103790-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NanoLuc luciferase with a nuclear localization signal (NLS) driven 
            by MET17 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB648
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..37
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        88..437
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     438..454
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     438..454
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             483..503
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             507..1019
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctccggat
       61 gcaagggttc gaatccctta gctctcatta ttttttgctt tttctcttga ggtcacatga
      121 tcgcaaaatg gcaaatggca cgtgaagctg tcgatattgg ggaactgtgg tggttggcaa
      181 atgactaatt aagttagtca aggcgccatc ctcatgaaaa ctgtgtaaca taataaccga
      241 agtgtcgaaa aggtggcacc ttgtccaatt gaacacgctc gatgaaaaaa ataagatata
      301 tataaggtta agtaaagcgt ctgttagaaa ggaagttttt cctttttctt gctctcttgt
      361 cttttcatct actatttcct tcgtgtaata cagggtcgtc agatacatag atacaattct
      421 attaccccca tccatactct agaactagtg gatcccccgg gttaattaac accatggcac
      481 caccaaagaa aaagagaaaa gtagccatgg tcttcacact cgaagatttc gttggggact
      541 ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt
      601 tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa
      661 atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa
      721 tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg
      781 tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt
      841 tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga
      901 ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc
      961 tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcgg
     1021 gtgggggatc attgtacaaa taaggcgcgc cacttctaaa taagcgaat
//
