LOCUS       sequence-103791-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disintegrator plasmid encoding MET17 promoter driven NanoLuc 
            (Promega) with nuclear localization signal (NLS) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB330
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(611..798)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     719..743
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
       61 cgcgttggcc gattcattaa tgcaggaccc gggagatcct ctctctgatc accgcatcat
      121 ccaaggatag tttacctgat ccaactaaga acttgggctg gtgcgatttc gtggggtgta
      181 ttcacgacat tttccaggac aatgctgaag ccttcccaga gagaacctgt gttgtggaga
      241 ctccaacact aaattccgac aagtcccgtt ctttcactta tcgcgacatc aaccgcactt
      301 ctaacatagt tgcccattat ttgattaaaa caggtatcaa aagaggtgat gtagtgatga
      361 tctattcttc taggggtgtg gatttgatgg tatgtgtgat gggtgtcttg aaagccggcg
      421 caaccttttc agttatcgac cctgcatatc ccccagccag acaaaccatt tacttaggtg
      481 ttgctaaacc acgtgggttg attgttatta gagctgctgg acaattggat caactagtag
      541 aagattacat caatgatgct ggcggccgcc tcgagagatc tatattaccc tgttatccct
      601 agcggatctg ccggtagagg tgtggtcaat aagagcgacc tcatactata cctgagaaag
      661 caacctgacc tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga
      721 gtcactttaa aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat
      781 aaatcataag aaattcgctt atttagaagt ggcgcgcctt atttgtacaa tgatccccca
      841 cccgccagaa tgcgttcgca cagccgccag ccggtcactc cgttgatggt tactcggaac
      901 agcagggagc cgtcggggtt gatcaggcgc tcgtcgataa ttttgttgcc gttccacagg
      961 gtccctgtta cagtgatctt tttgccgtcg aacacggcga tgccttcata cggccgtccg
     1021 aaatagtcga tcatgttcgg cgtaaccccg tcgattacca gtgtgccata gtgcaggatc
     1081 accttaaagt gatgatcatc
//
