LOCUS       sequence-103793-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disintegrator plasmid encoding MET17 promoter driven NanoLuc 
            (Promega) with nuclear localization signal (NLS) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB330
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(86..598)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(602..622)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(646..995)
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtggcgcgc
       61 cttatttgta caatgatccc ccacccgcca gaatgcgttc gcacagccgc cagccggtca
      121 ctccgttgat ggttactcgg aacagcaggg agccgtcggg gttgatcagg cgctcgtcga
      181 taattttgtt gccgttccac agggtccctg ttacagtgat ctttttgccg tcgaacacgg
      241 cgatgccttc atacggccgt ccgaaatagt cgatcatgtt cggcgtaacc ccgtcgatta
      301 ccagtgtgcc atagtgcagg atcaccttaa agtgatgatc atccacaggg tacaccacct
      361 taaaaatttt ttcgatctgg cccatttggt cgccgctcag accttcatac gggatgatga
      421 catggatgtc gatcttcagc ccattttcac cgctcaggac aatcctttgg atcggagtta
      481 cggacacccc gagattctga aacaaactgg acacacctcc ctgttcaagg acttggtcca
      541 ggttgtagcc ggctgtctgt cgccagtccc caacgaaatc ttcgagtgtg aagaccatgg
      601 ctacttttct ctttttcttt ggtggtgcca tggtgttaat taagagtatg gatgggggta
      661 atagaattgt atctatgtat ctgacgaccc tgtattacac gaaggaaata gtagatgaaa
      721 agacaagaga gcaagaaaaa ggaaaaactt cctttctaac agacgcttta cttaacctta
      781 tatatatctt atttttttca tcgagcgtgt tcaattggac aaggtgccac cttttcgaca
      841 cttcggttat tatgttacac agttttcatg aggatggcgc cttgactaac ttaattagtc
      901 atttgccaac caccacagtt ccccaatatc gacagcttca cgtgccattt gccattttgc
      961 gatcatgtga cctcaagaga aaaagcaaaa aataatgaga gctaagggat tcgaaccctt
     1021 gcatccggag ctcgaattcc agcctctgca agcgggctca ctggt
//
