LOCUS       sequence-103799-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) driven by SIC1 promoter.  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB767
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..36
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        18..36
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(409..427)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctcctccgg
       61 cgatcaaaca taccattccc ccctcaaatc aatatgatta agtcacgata atatctgcac
      121 tattgccgtg tttccttctc catcgacagc gtcgatcctg tctattatag ccagcacact
      181 gaaaatttca atggctggct tgttgttcgc gtcgcaactt ttgatattta taaaaagaga
      241 gaaggcatat ccaaaggata aggtattgtc tttgtaaatc aatcaaccaa acctctacgg
      301 aattttgacc cttgaagcag ggactattac acgttaatta acatggaaga cgtcaaaaac
      361 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
      421 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
      481 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
      541 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
      601 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
      661 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
      721 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
      781 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
      841 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
      901 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
      961 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
     1021 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     1081 tt
//
