LOCUS       sequence-10380-e        6695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Zhang Q, Gu J, Li L, Liu J, Luo B, Cheung HW, Boehm JS, Ni M, Geisen
            C, Root DE, Polyak K, Brown M, Richardson AL, Hahn WC, Kaelin WG, 
            Bommi-Reddy A
  TITLE     Control of Cyclin D1 and Breast Tumorigenesis by the EglN2 Prolyl 
            Hydroxylase.
  JOURNAL   Cancer Cell. 2009 Nov 6. 16(5):413-24.
  PUBMED    19878873
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2009 Nov 6. 16(5):413-24."
COMMENT     Sequence Label: cyclin D1-HA-pBabe-Hygro
FEATURES             Location/Qualifiers
     source          1..6695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             98..567
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    632..831
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1024..1440
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1408..1424
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      2225..2234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2512..2538
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2615..2635)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2620..2949
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2800..2935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3023..4048
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3023..4048
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             4843..5312
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5797..6588,6589..6657))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6420..6439
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6658..6695,1..67))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
      661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     1021 tagggccaga ctgttaccac tcccttaagt ttgaccttag atcactggaa agatgtcgag
     1081 cggctcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
     1141 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
     1201 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
     1261 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
     1321 gtacacccta agcctccgcc tcctcttctt ccatccgcgc cgtctctccc ccttgaacct
     1381 cctctttcga ccccgcctca atcctccctt tatccagccc tcactccttc tctaggcgcc
     1441 ggccggatcc agtgtggtgg tacgtaggaa ttcgggggcg cagtagcagc gagcagcaga
     1501 gtccgcacgc tccggcgagg ggcagaagag cgcgagggag cgcggggcag cagaagcgag
     1561 agccgagcgc ggacccagcc aggacccaca gccctcccca gctgcccagg aagagcccca
     1621 gccatggaac accagctcct gtgctgcgaa gtggaaacca tccgccgcgc gtaccccgat
     1681 gccaacctcc tcaacgaccg ggtgctgcgg gccatgctga aggcggagga gacctgcgcg
     1741 ccctcggtgt cctacttcaa atgtgtgcag aaggaggtcc tgccgtccat gcggaagatc
     1801 gtcgccacct ggatgctgga ggtctgcgag gaacagaagt gcgaggagga ggtcttcccg
     1861 ctggccatga actacctgga ccgcttcctg tcgctggagc ccgtgaaaaa gagccgcctg
     1921 cagctgctgg gggccacttg catgttcgtg gcctctaaga tgaaggagac catccccctg
     1981 acggccgaga agctgtgcat ctacaccgac aactccatcc ggcccgagga gctgctgcaa
     2041 atggagctgc tcctggtgaa caagctcaag tggaacctgg ccgcaatgac cccgcacgat
     2101 ttcattgaac acttcctctc caaaatgcca gaggcggagg agaacaaaca gatcatccgc
     2161 aaacacgcgc agaccttcgt tgccctctgt gccacagatg tgaagttcat ttccaatccg
     2221 ccctccatgg tggcagcggg gagcgtggtg gccgcagtgc aaggcctgaa cctgaggagc
     2281 cccaacaact tcctgtccta ctaccgcctc acacgcttcc tctccagagt gatcaagtgt
     2341 gacccagact gcctccgggc ctgccaggag cagatcgaag ctctgctgga gtcaagcctg
     2401 cgccaggccc agcagaacat ggaccccaag gccgccgagg aggaggaaga ggaggaggag
     2461 gaggtggacc tggcttgcac acccaccgac gtgcgggacg tggacatcgg ctacccctac
     2521 gacgtcccgg actacgccga tatctgatcc ggagcatttt gataccagaa gggaaagttg
     2581 gcggccgctc gagcatgcat tttgtcgacc ctgtggaatg tgtgtcagtt agggtgtgga
     2641 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2701 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2761 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2821 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2881 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2941 ttttgcaaaa agcttgccag ctggggcgcc ctctggtaag gttgggaagc cctgcagatc
     3001 tgatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     3061 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     3121 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     3181 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     3241 gattccggaa gtgcttgaca ttggggaatt tagcgagagc ctgacctatt gcatctcccg
     3301 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     3361 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     3421 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     3481 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     3541 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     3601 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     3661 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     3721 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     3781 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     3841 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     3901 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     3961 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     4021 cagcactcgt ccgagggcaa aggaatagag tagatgccga ccgggatcta tcgatgcggc
     4081 gggtgtggtg gttacgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4141 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4201 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4261 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4321 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4381 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4441 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4501 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4561 caggggaaac gcctggtatc ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4621 cgattttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcatcga
     4681 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     4741 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     4801 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
     4861 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
     4921 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4981 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
     5041 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     5101 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     5161 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     5221 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     5281 gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg
     5341 ttttttttct taagtattta cattaaatgg ccatagtgcg gccgcaaatc aatctaaagt
     5401 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     5461 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     5521 atacgggagg gcttaccatc tggcccccca aggcggtaat acggttatcc acagaatcag
     5581 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5641 aggccgcgtt acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5701 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagtttgcg gccgcaaatc
     5761 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5821 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5881 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5941 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6001 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6061 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6121 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6181 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6241 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6301 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6361 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     6421 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6481 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6541 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6601 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6661 cttccttttt caatattatt gaagcattta tcagg
//
