LOCUS       sequence-103801-         355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNA-Guided, Nuclease-Inactive 
            VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation 
            of Endogenous Genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Chakraborty S, Ji H, Kabadi AM, Gersbach CA, Christoforou N, Leong 
            KW
  TITLE     A CRISPR/Cas9-Based System for Reprogramming Cell Lineage 
            Specification.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):940-7. doi: 
            10.1016/j.stemcr.2014.09.013. Epub 2014 Oct 23.
  PUBMED    25448066
REFERENCE   2  (bases 1 to 355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.09.013"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "940-7"
COMMENT     Sequence Label: TetO-FUW-VdC9BV
FEATURES             Location/Qualifiers
     source          1..355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..241
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             248..271
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
       61 attgctagcc accatgggca gtgggagagc cgacgccctt gacgatttcg atctcgatat
      121 gttgggtagc gacgccctcg acgacttcga cctggatatg ctgggctcag atgcattgga
      181 tgacttcgat ctggacatgc tgggatcaga cgcgctggac gactttgacc tggatatgct
      241 catcaacgac tataaggacg atgacgataa gggaggtgga ggaagcggcg gtggaggcag
      301 cgggggcggt ggctctatgg ataagaaata ctctatcgga ctcgccattg gcaca
//
