LOCUS       sequence-103802-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNA-Guided, Nuclease-Inactive 
            VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation 
            of Endogenous Genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Chakraborty S, Ji H, Kabadi AM, Gersbach CA, Christoforou N, Leong 
            KW
  TITLE     A CRISPR/Cas9-Based System for Reprogramming Cell Lineage 
            Specification.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):940-7. doi: 
            10.1016/j.stemcr.2014.09.013. Epub 2014 Oct 23.
  PUBMED    25448066
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.09.013"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "940-7"
COMMENT     Sequence Label: TetO-FUW-VdC9BV
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..256)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(272..295)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(305..325)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(383..403)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tgtcagacct gaatttggtg cccatcctgg tctcgagtta gttaatcagc atgtccaggt
      121 cgaaatcatc aagggcgtca ctgccgagca tgtcgaggtc aaagtcatca agggcatccg
      181 aaccaagcat gtcaaggtca aaatcatcga gggcgtcact tcccagcata tccagatcaa
      241 aatcgtccaa tgcgtcagcc cgtccggaac ctttatcgtc gtcatccttg tagtcgctgg
      301 cctccacctt tctcttcttc ttggggctgc tacctccgcc accacttcca ccgcctccag
      361 aacctcctcc accggaacta cctaccttac gcttcttctt tggagctccc tcattaagct
      421 tgtgccccag tttgctaggg aggtcgcagt atctggccac tgccacctcg tgctgctcga
      481 cgtaggtctc gttgttggcc tccttgattc tttccagtct gtagtccaca tagtagacgc
      541 caggcatctt gaggttctta gcgggtttct tggatctata tgtggtcttg atgtttgcga
      601 tcagatggct cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc aggccgccgt
      661 ca
//
