LOCUS       sequence-103803-         536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes cytoplasmic cerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-C
FEATURES             Location/Qualifiers
     source          1..536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(10..43)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggatctagga taacttcgta taggatactt tatacgaagt tattgatcaa ttcacagcca
       61 ccatgggagc gccagctgga ggtgcagcaa tggtgtctaa aggagaggaa ctgtttaccg
      121 gagtggtccc aattctcgtg gaactggatg gcgatgtgaa tggacataaa ttttctgtgt
      181 ctggagaggg agagggcgat gccacctacg gcaagctgac cctgaaattt atttgcacca
      241 ccggcaaact gccagtgcca tggcctaccc tggtgaccac cctgacctgg ggcgtgcaat
      301 gctttgccag gtacccagat catatgaaac aacatgattt ttttaaatct gccatgccag
      361 agggctacgt gcaggagagg accatttttt ttaaagatga tggcaattac aaaaccaggg
      421 ccgaggtgaa atttgagggc gataccctgg tgaataggat tgagctcaaa ggcattgatt
      481 ttaaagagga tggcaatatt ctgggacaca aactggagta caatgccatt tctgat
//
