LOCUS       sequence-103805-         547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes nuclear citrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-hY
FEATURES             Location/Qualifiers
     source          1..547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(10..43)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             63..437
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSA"
ORIGIN
        1 ggatctagga taacttcgta taggatactt tatacgaagt tattgatcaa ttcacagcca
       61 ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag aaggcggtga
      121 ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag agctattcca
      181 tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg tccaaggcca
      241 tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc
      301 gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag acggccgtgc
      361 gcctgctgct gcctggggag ttggccaagc acgccgtgtc cgagggtact aaggccatca
      421 ccaagtacac cagcgctgga gcgccagctg gaggtgcagg tgcagcaatg gtgtctaagg
      481 gcgaggagct gtttacaggc gtggtgccaa tcctcgtgga gctcgatggc gatgtgaacg
      541 gccataa
//
