LOCUS       sequence-103808-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdN13 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-466
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             64..86
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             757..774
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(814..831)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagaaat
       61 aattttgttt aactttaaga aggagatata catatgactg taccacacat ggaatcaatt
      121 gaggagatgt atagtaaata cgcctcgatg aatggggaat taccattcga taatggttac
      181 gcagttccat tagataatgt ctttgtgtac acgttggaca tcgctagtgg tgaaattaag
      241 aaaacgaggg cttcatacat ctatcgcgag aaggttgaga agctcattga gataaaacta
      301 tccagtggat actcccttaa ggttactccc tctcacccag ttctcctctt tagagatggc
      361 ctgcaatggg tccccgctgc tgaagttaaa cctggagatg ttgttgttgg tgttagggaa
      421 gaggtactga gaagaagaat aatatccaaa ggagaacttg aattccatga ggtttcctcg
      481 gtaaggataa tagattacaa taactgggtc tacgaccttg taattccgga aacccacaac
      541 ttcatagctc ccaatggact tgttctccat aatactcagc taggtaccca gtacaaactt
      601 atcctgaacg gtaaaaccct gaaaggtgaa accaccaccg aagctgttga cgctgctacc
      661 gcggaaaaag ttttcaaaca gtacgctaac gacaacggtg ttgacggtga atggacctac
      721 gacgacgcta ccaaaaccta caccgtaacg gaattccatc atcatcatca tcattaagct
      781 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
      841 aagcggtctg ataaaa
//
