LOCUS       sequence-103809-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdN13 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-466
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             699..721
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccaagctta atgatgatga tgatgatgga attccgttac ggtgtaggtt ttggtagcgt
       61 cgtcgtaggt ccattcaccg tcaacaccgt tgtcgttagc gtactgtttg aaaacttttt
      121 ccgcggtagc agcgtcaaca gcttcggtgg tggtttcacc tttcagggtt ttaccgttca
      181 ggataagttt gtactgggta cctagctgag tattatggag aacaagtcca ttgggagcta
      241 tgaagttgtg ggtttccgga attacaaggt cgtagaccca gttattgtaa tctattatcc
      301 ttaccgagga aacctcatgg aattcaagtt ctcctttgga tattattctt cttctcagta
      361 cctcttccct aacaccaaca acaacatctc caggtttaac ttcagcagcg gggacccatt
      421 gcaggccatc tctaaagagg agaactgggt gagagggagt aaccttaagg gagtatccac
      481 tggatagttt tatctcaatg agcttctcaa ccttctcgcg atagatgtat gaagccctcg
      541 ttttcttaat ttcaccacta gcgatgtcca acgtgtacac aaagacatta tctaatggaa
      601 ctgcgtaacc attatcgaat ggtaattccc cattcatcga ggcgtattta ctatacatct
      661 cctcaattga ttccatgtgt ggtacagtca tatgtatatc tccttcttaa agttaaacaa
      721 aattatttct agcccaaaaa aacgggtatg gagaaacagt agagagttgc
//
