LOCUS       sequence-103810-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaB(delta283)dN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-111
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             64..86
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             682..699
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(739..756)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagaaat
       61 aattttgttt aactttaaga aggagatata catatgggat ccggactgca agtgccaatt
      121 aaggaattag taggtaaatc tggttttgca gtttgggcat tgaacgaagc tacaatgcag
      181 ctagaaaagg caattgttag caatgctttt tcgactggta taaagcctct gtttactttg
      241 acaactcgat tggggcggaa aatccgtgcc acaggtaatc acaagttttt aacaattaat
      301 ggctggaaaa gacttgatga attaactcct aaagaacatc tcgctttacc aagaaatctc
      361 cccagttctg gtactcttgc caaaagtgat atttattggg atgaaatagt ttcaattgaa
      421 tatagtggtg aggaagaagt gtttgacctc accgttcctg gtttgcataa ttttgttgca
      481 aataatatta ttgttcacaa ttccattggt acccagtaca aacttatcct gaacggtaaa
      541 accctgaaag gtgaaaccac caccgaagct gttgacgctg ctaccgcgga aaaagttttc
      601 aaacagtacg ctaacgacaa cggtgttgac ggtgaatgga cctacgacga cgctaccaaa
      661 acctacaccg taacggaatt ccatcatcat catcatcatt aagcttggct gttttggcgg
      721 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
      781 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
      841 agtgaaacgc cgtagcgcc
//
