LOCUS       sequence-103812-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAC14 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-167
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             60..82
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             318..335
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(375..392)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      545..631
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      724..751
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agaaataatt
       61 ttgtttaact ttaagaagga gatatacata tgacccacaa cttcatagct cccaatggac
      121 ttgttctcca taatactcag ctaggtaccc agtacaaact tatcctgaac ggtaaaaccc
      181 tgaaaggtga aaccaccacc gaagctgttg acgctgctac cgcggaaaaa gttttcaaac
      241 agtacgctaa cgacaacggt gttgacggtg aatggaccta cgacgacgct accaaaacct
      301 acaccgtaac ggaattccat catcatcatc atcattaagc ttggctgttt tggcggatga
      361 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
      421 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
      481 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
      541 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      601 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
      661 gcaacggccc ggaggggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
      721 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
      781 ttctaaatac att
//
