LOCUS       sequence-103816-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive repeat-only E. coli CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Luo ML, Mullis AS, Leenay RT, Beisel CL
  TITLE     Repurposing endogenous type I CRISPR-Cas systems for programmable 
            gene repression.
  JOURNAL   Nucleic Acids Res. 2014 Oct 17. pii: gku971.
  PUBMED    25326321
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Oct 17. pii: gku971."
COMMENT     Sequence Label: pcrRNA.con
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     105..124
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        219..253
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     complement(389..406)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      559..645
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
       61 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      121 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      181 aggatctgct catgtttgac agcttatcat cgatgcattt gacagctagc tcagtcctag
      241 gtataatgct agcgctagcg aattcgagct cggtaccacc tcgagttccc cgcgccagcg
      301 gggataaacc gaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt aagcttggct
      361 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
      421 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
      481 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
      541 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      601 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
      661 ttgaacgttg cgaagcaacg gcccgga
//
